The annexin valexa fluor 647 life technologies, grand island, ny staining procedure. We have 1 invitrogen qubit 4 manual available for free pdf download. Download acrobat pdf file 487kb supplementary material. Mitochondrial staining allows robust elimination of apoptotic and. R123 and all other chemicals were from sigma chemical co. Tetramethylrhodamine, methyl ester, perchlorate tmrm. Tmre mitochondrial membrane potential assay gbiosciences. Mitotracker green fm is greenfluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential. Age is a nonmodifiable risk factor for the inflammation that underlies ageassociated diseases. Preferably store it undiluted in small aliquots at 20c. Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential. Rh123, sigma, usa was dissolved with dmso to 10 mgml for storage at 80 c, tetramethylrhodamine ethyl ester tmre invitrogen, molecular probes, usa was diluted with dmso to 1 mmoll for storage at 80 c. Tetramethylrhodamine tmrm thermo fisher scientific.
Invitrogen tetramethylrhodamine, ethyl ester, perchlorate. This protocol describes labeling of mitochondria with tmrm or tmre. Tetramethylrhodamine, ethyl ester tmre is a cellpermeant, cationic, redorange fluorescent dye that is readily sequestered by active mitochondria. Detection of apoptosis in sknmc neuroblastoma cells. Inhibition of respiration was greatest with tmre, followed by r123 and tmrm. Measurement of mitochondrial membrane potential using.
During oxidative phosphorylation, the gradual degradation of molecules through the. Download pdf main to study metabolic adaptation of fh deficient cells we first generated immortalized kidney cells from mice homozygous for a conditionally targeted fh1 allele 3. Pdf usage of a localised microflow device to show that. Tmre is a cellpermeant and mitochondria membranepermeant, redorange fluorescent. Flow cytometric analysis of tmre staining in jurkat cells. Vector nti download archive thermo fisher scientific us. Invitrogen tetramethylrhodamine, ethyl ester, perchlorate tmre 25mg life sciences.
Functional mitochondrial staining protocol this protocol provides basic instructions to perform a. Instructions mper mammalian protein extraction reagent. Metformin enhances autophagy and normalizes mitochondrial. Tetramethylrhodamine methyl ester tmrm life technologies, catalog. Continued clearance of apoptotic cells critically depends. Mini dls for windows recommended for serving up to five access licenses. Invitrogen qubit 4 manuals manuals and user guides for invitrogen qubit 4. Usage of a localised microflow device to show that mitochondrial networks are not extensive in skeletal muscle fibres.
Tetramethylrhodamine, ethyl ester, perchlorate tmre. Mitochondrial membrane potential was also measured using tmre invitrogen or a dual emission potentialsensitive. Tetramethylrhodamine, methyl ester tmrm is a cellpermeant, cationic, redorange fluorescent dye that is readily sequestered by active mitochondria. Tetramethylrhodamine, methyl ester tmrm is a cellpermeant dye that. Labeling mitochondria with tmrm or tmre article in cold spring harbor protocols 20117. Download pdf introduction in vivo twophoton imaging is an analytical approach that can be used to visualize cell dynamics and hemodynamics in organs or tissues of live animals. Translation initiator eif4g1 mutations in familial. The dye will stain live cells but is not wellretained after aldehyde fixation. Labeling mitochondria with tmrm or tmre request pdf. Tetramethylrhodamine tmrm catalog number i34361, t668 pub.
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